GSBS Student Publications

Title

Mutation of glycine 49 to valine in the alpha subunit of GS results in the constitutive elevation of cyclic AMP synthesis

GSBS Program

Not applicable

Publication Date

1989-05-30

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The G-protein GS couples hormone-activated receptors with adenylyl cyclase and stimulates increased cyclic AMP synthesis. Transient expression in COS-1 cells of cDNAs coding for the GS alpha-subunit (alpha S) or alpha S cDNAs having single amino acid mutations Gly49----Val or Gly225----Thr elevated cyclic AMP levels, resulting in the activation of cyclic AMP dependent protein kinase. Stable expression in Chinese hamster ovary cells of alpha S Val49 cDNA resulted in a small constitutive elevation of cyclic AMP that was sufficient to persistently activate cyclic AMP dependent protein kinase activity 1.5-2-fold over basal activity. Stable expression of wild-type alpha S or alpha S Thr225 in Chinese hamster ovary cells was less effective in sustaining elevated cyclic AMP synthesis and kinase activation compared to alpha SVal49.

DOI of Published Version

10.1021/bi00437a006

Source

Biochemistry. 1989 May 30;28(11):4547-51.

Journal/Book/Conference Title

Biochemistry

Related Resources

Link to Article in PubMed

PubMed ID

2548581

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