Class VI myosin moves processively along actin filaments backward with large steps
Graduate School of Biomedical Sciences; Department of Physiology
Medical Subject Headings
Actins; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Cell Line; DNA, Complementary; Green Fluorescent Proteins; Insects; Luminescent Proteins; Microscopy, Electron; Models, Biological; Muscle, Skeletal; Myosin Heavy Chains; Myosin Type V; Protein Binding; Rabbits; Recombinant Fusion Proteins; Recombinant Proteins; Time Factors; Xenopus
Life Sciences | Medicine and Health Sciences
Among a superfamily of myosin, class VI myosin moves actin filaments backwards. Here we show that myosin VI moves processively on actin filaments backwards with large ( approximately 36 nm) steps, nevertheless it has an extremely short neck domain. Myosin V also moves processively with large ( approximately 36 nm) steps and it is believed that myosin V strides along the actin helical repeat with its elongated neck domain that is critical for its processive movement with large steps. Myosin VI having a short neck cannot take this scenario. We found by electron microscopy that myosin VI cooperatively binds to an actin filament at approximately 36 nm intervals in the presence of ATP, raising a hypothesis that the binding of myosin VI evokes "hot spots" on actin filaments that attract myosin heads. Myosin VI may step on these "hot spots" on actin filaments in every helical pitch, thus producing processive movement with 36 nm steps.
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Citation: Biochem Biophys Res Commun. 2002 Jan 11;290(1):311-7. Link to article on publisher's site
DOI of Published Version
Biochemical and biophysical research communications
Nishikawa, So; Homma, Kazuaki; Komori, Yasunori; Iwaki, Mitsuhiro; Wazawa, Tetsuichi; Iwane, Atsuko Hikikoshi; Saito, Junya; Ikebe, Reiko; Katayama, Eisaku; Yanagida, Toshio; and Ikebe, Mitsuo, "Class VI myosin moves processively along actin filaments backward with large steps" (2002). GSBS Student Publications. 902.