Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryos
Graduate School of Biomedical Sciences; Program in Molecular Medicine
Life Sciences | Medicine and Health Sciences
beta-Catenin regulates cell adhesion and cellular differentiation during development, and misregulation of beta-catenin contributes to numerous forms of cancer in humans. Here we describe Caenorhabditis elegans conditional alleles of mom-2/Wnt, mom-4/Tak1, and wrm-1/beta-catenin. We use these reagents to examine the regulation of WRM-1/beta-catenin during a Wnt-signaling-induced asymmetric cell division. While WRM-1 protein initially accumulates in the nuclei of all cells, signaling promotes the retention of WRM-1 in nuclei of responding cells. We show that both PRY-1/Axin and the nuclear exportin homolog IMB-4/CRM-1 antagonize signaling. These findings reveal how Wnt signals direct the asymmetric localization of beta-catenin during polarized cell division.
DOI of Published Version
Genes Dev. 2005 Aug 1;19(15):1749-54. Link to article on publisher's site
Genes and development
Nakamura T, Kim S, Ishidate T, Bei Y, Pang KM, Shirayama M, Trzepacz C, Brownell DR, Mello CC. (2005). Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryos. GSBS Student Publications. https://doi.org/10.1101/gad.1323705. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/895