GSBS Student Publications
Title
A novel injectable approach for cartilage formation in vivo using PLG microspheres
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Cell Biology
Date
4-21-2004
Document Type
Article
Medical Subject Headings
Animals; Biocompatible Materials; Cartilage, Articular; Cattle; Cell Division; Cell Survival; Cell Transplantation; Cells, Cultured; Chondrocytes; Feasibility Studies; Glycolates; Glycosaminoglycans; Injections; Materials Testing; Mice; Microspheres; Tissue Engineering
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
This study documents the use of biodegradable poly(lactide-co-glycolide) (PLG) microspheres as a novel, injectable scaffold for cartilage tissue engineering. Chondrocytes were delivered via injection to the subcutaneous space of athymic mice in the presence and absence of PLG microspheres. Tissue formation was evaluated up to 8 weeks post-injection. Progressive cartilage formation was observed in samples containing microspheres. The presence of microspheres increased the quantity of tissue formed, the amount of glycosaminoglycan that accumulated, and the uniformity of type II collagen deposition. Microsphere composition influenced the growth of the tissue engineered cartilage. Higher molecular weight PLG resulted in a larger mass of cartilage formed and a higher content of proteoglycans. Microspheres comprised PLG with methyl ester end groups yielded increased tissue mass and matrix accumulation, but did not display homogenous matrix deposition. The microencapsulation of Mg(OH)2 had negative effects on tissue mass and matrix accumulation. Matrix accumulation, cell number, and tissue mass were unchanged by microsphere size, but larger microspheres increased the frequency of central necrosis in implants. The data herein reflect the promising utility of an injectable PLG-chondrocyte system for tissue engineering applications.
Rights and Permissions
Citation: Ann Biomed Eng. 2004 Mar;32(3):418-29.
Related Resources
Journal Title
Annals of biomedical engineering
PubMed ID
15095816
Repository Citation
Mercier, Nichole Renee; Costantino, Henry R.; Tracy, Mark A.; and Bonassar, Lawrence J., "A novel injectable approach for cartilage formation in vivo using PLG microspheres" (2004). GSBS Student Publications. 855.
https://escholarship.umassmed.edu/gsbs_sp/855