A novel injectable approach for cartilage formation in vivo using PLG microspheres
Document Type
Journal ArticlePublication Date
2004-04-21Keywords
Animals; Biocompatible Materials; Cartilage, Articular; Cattle; Cell Division; Cell Survival; Cell Transplantation; Cells, Cultured; Chondrocytes; Feasibility Studies; Glycolates; Glycosaminoglycans; Injections; Materials Testing; Mice; Microspheres; Tissue EngineeringLife Sciences
Medicine and Health Sciences
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Show full item recordAbstract
This study documents the use of biodegradable poly(lactide-co-glycolide) (PLG) microspheres as a novel, injectable scaffold for cartilage tissue engineering. Chondrocytes were delivered via injection to the subcutaneous space of athymic mice in the presence and absence of PLG microspheres. Tissue formation was evaluated up to 8 weeks post-injection. Progressive cartilage formation was observed in samples containing microspheres. The presence of microspheres increased the quantity of tissue formed, the amount of glycosaminoglycan that accumulated, and the uniformity of type II collagen deposition. Microsphere composition influenced the growth of the tissue engineered cartilage. Higher molecular weight PLG resulted in a larger mass of cartilage formed and a higher content of proteoglycans. Microspheres comprised PLG with methyl ester end groups yielded increased tissue mass and matrix accumulation, but did not display homogenous matrix deposition. The microencapsulation of Mg(OH)2 had negative effects on tissue mass and matrix accumulation. Matrix accumulation, cell number, and tissue mass were unchanged by microsphere size, but larger microspheres increased the frequency of central necrosis in implants. The data herein reflect the promising utility of an injectable PLG-chondrocyte system for tissue engineering applications.Source
Ann Biomed Eng. 2004 Mar;32(3):418-29.
DOI
10.1023/B:ABME.0000017547.84146.fdPermanent Link to this Item
http://hdl.handle.net/20.500.14038/34198PubMed ID
15095816Related Resources
ae974a485f413a2113503eed53cd6c53
10.1023/B:ABME.0000017547.84146.fd