Temporal onset of synapsin I gene expression coincides with neuronal differentiation during the development of the nervous system
Graduate School of Biomedical Sciences; Department of Neurology
Medical Subject Headings
Animals; Brain; Cell Differentiation; Cerebellum; Gene Expression; Hippocampus; In Situ Hybridization; Neurons; Peripheral Nervous System; RNA, Messenger; Rats; Rats, Sprague-Dawley; Synapsins; Time Factors
Life Sciences | Medicine and Health Sciences
Synapsin I is the best characterized member of a family of nerve terminal-specific phosphoproteins implicated in the regulation of neurotransmitter release. During development, the expression of synapsin I correlates temporally and topographically with synapse formation, and recent physiological studies (Lu et al.  Neuron 8:521-529.) have suggested that synapsin I may participate in the functional maturation of synapses. To better understand the temporal relationship between synapsin I gene expression and particular cellular events during neuronal development, we have used in situ hybridization histochemistry to localize synapsin I mRNA throughout the rat central and peripheral nervous systems during embryonic and postnatal development. From the earliest embryonic time points assayed (E12), the expression of the synapsin I gene was detectable in both the central and peripheral nervous systems. While, in general, levels of synapsin I mRNAs were high in utero, synapsin I cDNA probes revealed specific patterns of hybridization in different regions of the embryonic nervous system. To determine precisely the temporal onset of expression of the synapsin I gene during neuronal development, we examined in detail the appearance of synapsin I mRNA during the well characterized postnatal development of granule cells of the rat cerebellum and hippocampus. In both regions, the onset of synapsin I gene expression correlated with the period of stem cell commitment to terminal differentiation. Finally, our data demonstrate that, in a second phase, synapsin I gene expression increases to a maximum for a given neuronal population during a particular phase of differentiation, i.e., synaptogenesis.
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Citation: J Comp Neurol. 1994 Apr 15;342(3):449-62. Link to article on publisher's site
DOI of Published Version
The Journal of comparative neurology
Melloni, Richard H. and DeGennaro, Louis J., "Temporal onset of synapsin I gene expression coincides with neuronal differentiation during the development of the nervous system" (1994). GSBS Student Publications. 852.