Identification of murine poxvirus-specific CD8+ CTL epitopes with distinct functional profiles
Center for Infectious Disease and Vaccine Research
Medical Subject Headings
Amino Acid Sequence; Animals; Cell Degranulation; Cell Line, Tumor; Cells, Cultured; *Cytotoxicity Tests, Immunologic; Epitopes, T-Lymphocyte; H-2 Antigens; Injections, Intraperitoneal; Interferon Type II; Kinetics; Male; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Peptide Fragments; T-Lymphocytes, Cytotoxic; Vaccinia virus; Viral Vaccines
Life Sciences | Medicine and Health Sciences
Murine T cell epitopes against vaccinia virus (VV) have not been characterized to date in part due to the large and complex genome of VV. We have identified and characterized two CD8+ T cell epitopes on the A47L (modified VV Ankara strain (MVA)-029) and J6R (MVA-043) proteins of VV that are Db and Kb restricted, respectively. Following i.p. immunization with VV New York City Board of Health (NYCBH) strain, MVA-029 peptide-stimulated splenocytes secreted IFN-gamma from 7 days to 7 mo postimmunization, and virus-stimulated effectors were also able to lyse MVA-029-pulsed target cells at the same time points. In contrast, MVA-043 peptide-stimulated splenocytes secreted very low levels of IFN-gamma only at day 7 but maintained the ability to lyse target cells up to 2 mo postimmunization. Both MVA-029 and MVA-043 peptide-stimulated lymph node cells degranulated similarly as assessed by Ag-induced CD107 expression. T cell responses to whole-virus stimulation remained robust and steady during the acute and memory T cell response to VV. Identification of T cell epitopes on VV will enable further studies to increase our understanding of the role of CD8+ T cells in VV infection and assist in the design of new protective strategies.
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Citation: J Immunol. 2005 Feb 15;174(4):2212-9.
Journal of immunology (Baltimore, Md. : 1950)
Mathew, Anuja; Terajima, Masanori; West, Kim; Green, Sharone; Rothman, Alan L.; Ennis, Francis A.; and Kennedy, Jeffrey S., "Identification of murine poxvirus-specific CD8+ CTL epitopes with distinct functional profiles" (2005). GSBS Student Publications. 819.