myc and E1A oncogenes alter the responses of PC12 cells to nerve growth factor and block differentiation
Graduate School of Biomedical Sciences; Center for Cancer Research
Medical Subject Headings
Adenovirus Early Proteins; *Cell Differentiation; *Cell Division; DNA Replication; Enzyme Induction; Nerve Growth Factors; Oncogene Proteins, Viral; *Oncogenes; Ornithine Decarboxylase; Pheochromocytoma; Receptors, Cell Surface; Receptors, Nerve Growth Factor; Tumor Cells, Cultured
Life Sciences | Medicine and Health Sciences
PC12 rat pheochromocytoma cells respond to nerve growth factor (NGF) by neuronal differentiation and partial growth arrest. Mouse c-myc and adenovirus E1A genes were introduced into PC12 cells to study the influence of these nuclear oncogenes on neuronal differentiation. Expression of myc or E1A blocked morphological differentiation and caused NGF to stimulate rather than inhibit cell proliferation. NGF binding to cell surface receptors and ornithine decarboxylase induction were similar in myc- and E1A-expressing clones compared with wild-type PC12 cells, suggesting that changes in the cellular response to NGF were at a post-receptor level. These results illustrate that NGF can promote either growth or differentiation of PC12 cells, and that myc or E1A alter the phenotypic responses to growth factors and hormones.
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Citation: Oncogene. 1987;1(4):361-7.
Maruyama, Kazuo; Schiavi, Susan C.; Huse, William; Johnson, Gary L.; and Ruley, H. Earl, "myc and E1A oncogenes alter the responses of PC12 cells to nerve growth factor and block differentiation" (1987). GSBS Student Publications. 812.