B lymphocyte development and activation independent of MHC class II expression
Graduate School of Biomedical Sciences; Program in Immunology and Virology; Department of Cancer Biology
Medical Subject Headings
Animals; Antibody Formation; B-Lymphocytes; Cell Differentiation; Flow Cytometry; *Genes, MHC Class II; Histocompatibility Antigens Class II; Leukocyte Count; *Lymphocyte Activation; Lymphocyte Cooperation; Mice; Mutagenesis, Insertional; Receptors, Antigen, B-Cell; T-Lymphocytes
Life Sciences | Medicine and Health Sciences
A murine model of MHC class II deficiency created by targeted gene disruption was used to investigate whether class II expression influences B cell maturation and function. There appeared to be fewer total B cell precursors, a higher proportion of which were in a very early stage of maturation, in class II-deficient vs control bone marrow; however, the differences did not reach statistical significance. Mature B cells were unaffected; IgM, IgD, B220, and CD5 surface expression were similar in class II-deficient and control animals. Serum Ig determinations revealed that the class II-deficient animals had elevated IgM but decreased IgG1 (and, variably, IgE) compared to control. The antibody response against thymic-independent Ag was intact in class II-animals, as was the in vitro response of small resting B cells from class II deficient animals to stimulation with polyclonal B cell activators. Preactivated T cells were able to induce differentiation and proliferation of class II-deficient, small resting B cells. Together, these data indicate that B cell development, T cell-independent, and T cell-dependent B-cell activation, can occur independently of class II MHC expression.
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Citation: J Immunol. 1993 Feb 15;150(4):1223-33.
Journal of immunology (Baltimore, Md. : 1950)
Markowitz, Jay S.; Rogers, Paul R.; Grusby, Michael J.; Parker, David C.; and Glimcher, Laurie H., "B lymphocyte development and activation independent of MHC class II expression" (1993). GSBS Student Publications. 808.