GSBS Student Publications

Title

Mutation of the SNF2 family member Chd2 affects mouse development and survival

Publication Date

2006-07-01

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology; Department of Cancer Biology,

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The chromodomain helicase DNA-binding domain (Chd) proteins belong to the SNF2-like family of ATPases that function in chromatin remodeling and assembly. These proteins are characterized by the presence of tandem chromodomains and are further subdivided based on the presence or absence of additional structural motifs. The Chd1-Chd2 subfamily is distinguished by the presence of a DNA-binding domain that recognizes AT-rich sequence. Currently, there are no reports addressing the function of the Chd2 family member. Embryonic stem cells containing a retroviral gene-trap inserted at the Chd2 locus were utilized to generate mice expressing a Chd2 protein lacking the DNA-binding domain. This mutation in Chd2 resulted in a general growth delay in homozygous mutants late in embryogenesis and in perinatal lethality. Animals heterozygous for the mutation showed decreased neonatal viability and increased susceptibility to non-neoplastic lesions affecting most primary organs. In particular, approximately 85% of the heterozygotes showed gross kidney abnormalities. Our results demonstrate that mutation of Chd2 dramatically affects mammalian development and long-term survival.

DOI of Published Version

10.1002/jcp.20718

Source

J Cell Physiol. 2006 Oct;209(1):162-71. Link to article on publisher's site

Journal/Book/Conference Title

Journal of cellular physiology

Related Resources

Link to article in PubMed

PubMed ID

16810678

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