Regulation of mRNA stability and the poly(A) problem in Dictyostelium discoideum
Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology
Life Sciences | Medicine and Health Sciences
This paper reviews our studies of three aspects of post-transcriptional regulation in Dictyostelium discoideum: 1) the determinants of mRNA stability in vegetative amoebae; 2) the effects of disaggregation and cyclic AMP on the decay rates of cell-type-specific mRNAs in late developing cells; and 3) the cytoplasmic function of the 3' poly(A) tracts present on most mRNAs. We find that: 1) mRNA stability in vegetative amoebae is not dependent on mRNA size, ribosome loading, or poly(A) tract length, but may be determined by specific 3'-untranslated sequences within a given mRNA; 2) mRNA decay rates in late developing cells are heterogeneous, and cyclic AMP does not act directly to stabilize cell-type-specific mRNAs; and 3) poly(A) is most likely involved in the initiation of protein synthesis via an interaction with cytoplasmic poly(A)-binding proteins.
DOI of Published Version
Dev Genet. 1988;9(4-5):403-19. Link to article on publisher's site
Manrow RE, Shapiro RA, Herrick D, Steel LF, Blinder DB, Jacobson A. (1988). Regulation of mRNA stability and the poly(A) problem in Dictyostelium discoideum. GSBS Student Publications. https://doi.org/10.1002/dvg.1020090420. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/804