Regulation of mRNA stability and the poly(A) problem in Dictyostelium discoideum
Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology
Life Sciences | Medicine and Health Sciences
This paper reviews our studies of three aspects of post-transcriptional regulation in Dictyostelium discoideum: 1) the determinants of mRNA stability in vegetative amoebae; 2) the effects of disaggregation and cyclic AMP on the decay rates of cell-type-specific mRNAs in late developing cells; and 3) the cytoplasmic function of the 3' poly(A) tracts present on most mRNAs. We find that: 1) mRNA stability in vegetative amoebae is not dependent on mRNA size, ribosome loading, or poly(A) tract length, but may be determined by specific 3'-untranslated sequences within a given mRNA; 2) mRNA decay rates in late developing cells are heterogeneous, and cyclic AMP does not act directly to stabilize cell-type-specific mRNAs; and 3) poly(A) is most likely involved in the initiation of protein synthesis via an interaction with cytoplasmic poly(A)-binding proteins.
DOI of Published Version
Dev Genet. 1988;9(4-5):403-19. Link to article on publisher's site
Manrow, Richard E.; Shapiro, Robert A.; Herrick, David; Steel, Laura F.; Blinder, Dmitry B.; and Jacobson, Allan, "Regulation of mRNA stability and the poly(A) problem in Dictyostelium discoideum" (1988). GSBS Student Publications. 804.