Deficient human immunodeficiency virus type 1-specific cytotoxic T cell responses in vertically infected children
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Program in Immunology and Virology; Department of Pediatrics; Department of Medicine, Division of Hematology and Oncology
Life Sciences | Medicine and Health Sciences
Cytotoxic T lymphocyte (CTL) responses to human immunodeficiency virus type 1 (HIV-1) gag proteins were studied prospectively in 17 children (12 infected) born of mothers with HIV-1 seropositivity and in five pediatric patients with hemophilia infected by transfusion of HIV-1-contaminated factor VIII concentrate. B lymphoblastoid cells infected with vaccinia virus vectors expressing HIV-1 gag gene products were combined with autologous peripheral blood mononuclear cells to detect circulating CTLs. Effector cells were defined by monoclonal antibody-mediated, complement-dependent cytolysis. Circulating HIV-1 gag-specific cytotoxic responses were detectable in 4 of 5 HIV-1-infected pediatric hemophilic patients, and were similar in magnitude to those previously described in adults. In contrast, circulating HIV-1 gag-specific cytolysis was detectible in only 3 of 12 vertically infected children. Depletion data revealed that the majority of detectible gag-specific cytolysis was CD8 T cell-mediated. No apparent relationships between CD4 T cell counts, CD8 T cells counts, or serum p24 antigen levels and CTL responses were seen. Deficient CTL development may, in part, explain the more rapid onset of symptomatic disease following vertical HIV infection.
DOI of Published Version
J Pediatr. 1991 Aug;119(2):230-6.
The Journal of pediatrics
Luzuriaga, Katherine; Koup, Richard A.; Pikora, Cheryl A.; Brettler, Doreen B.; and Sullivan, John L., "Deficient human immunodeficiency virus type 1-specific cytotoxic T cell responses in vertically infected children" (1991). GSBS Student Publications. 794.