GSBS Student Publications
Title
The Tir-binding region of enterohaemorrhagic Escherichia coli intimin is sufficient to trigger actin condensation after bacterial-induced host cell signalling
GSBS Program
Biochemistry & Molecular Pharmacology
Publication Date
1999-12-14
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Enterohaemorrhagic Escherichia coli (EHEC) has emerged as an important agent of diarrhoeal disease. Attachment to host cells, an essential step during intestinal colonization by EHEC, is associated with the formation of a highly organized cytoskeletal structure containing filamentous actin, termed an attaching and effacing (A/E) lesion, directly beneath bound bacteria. The outer membrane protein intimin is required for the formation of this structure, as is Tir, a bacterial protein that is translocated into the host cell and is thought to function as a receptor for intimin. To understand intimin function better, we fused EHEC intimin to a homologous protein, Yersinia pseudotuberculosis invasin, or to maltose-binding protein. The N-terminal 539 amino acids of intimin were sufficient to promote outer membrane localization of the C-terminus of invasin and, conversely, the N-terminal 489 amino acids of invasin were sufficient to promote the localization of the C-terminus of intimin. The C-terminal 181 residues of intimin were sufficient to bind mammalian cells that had been preinfected with an enteropathogenic E. coli strain that expresses Tir but not intimin. Binding of intimin derivatives to preinfected cells correlated with binding to recombinant Tir protein. Finally, the 181-residue minimal Tir-binding region of intimin, when purified and immobilized on latex beads, was sufficient to trigger A/E lesions on preinfected mammalian cells.
DOI of Published Version
10.1046/j.1365-2958.1999.01574.x
Source
Mol Microbiol. 1999 Oct;34(1):67-81.
Journal/Book/Conference Title
Molecular microbiology
Related Resources
PubMed ID
10540286
Repository Citation
Liu H, Magoun L, Luperchio SA, Schauer DB, Leong JM. (1999). The Tir-binding region of enterohaemorrhagic Escherichia coli intimin is sufficient to trigger actin condensation after bacterial-induced host cell signalling. GSBS Student Publications. https://doi.org/10.1046/j.1365-2958.1999.01574.x. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/773