Title

BACH1 is critical for homologous recombination and appears to be the Fanconi anemia gene product FANCJ

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cancer Biology; Program in Molecular Medicine

Publication Date

2005-09-13

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We showed in this study that cells deficient of the BRCA1-associated BACH1 helicase, also known as BRIP1, failed to elicit homologous recombination (HR) after DNA double-stranded breaks (DSBs). BACH1-deficient cells were also sensitive to mitomycin C (MMC) and underwent MMC-induced chromosome instability. Moreover, we identified a homozygous nonsense mutation in BACH1 in a FA-J patient-derived cell line and could not detect BACH1 protein in this cell line. Expression of wild-type BACH1 in this cell line reduced the accumulation of cells at G2/M phases following exposure to DNA crosslinkers, a characteristic of Fanconi anemia (FA) cells. These results support the conclusion that BACH1 is FANCJ.

DOI of Published Version

10.1016/j.ccr.2005.08.004

Source

Cancer Cell. 2005 Sep;8(3):255-65. Link to article on publisher's site

Journal/Book/Conference Title

Cancer cell

Related Resources

Link to article in PubMed

PubMed ID

16153896

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