GSBS Student Publications


Higher level organization of individual gene transcription and RNA splicing

Publication Date


UMMS Affiliation

Department of Cell Biology; Department of Molecular Genetics and Microbiology

Document Type



Cell Biology | Life Sciences | Medicine and Health Sciences


Visualization of fibronectin and neurotensin messenger RNAs within mammalian interphase nuclei was achieved by fluorescence hybridization with genomic, complementary DNA, and intron-specific probes. Unspliced transcripts accumulated in one or two sites per nucleus. Fibronectin RNA frequently accumulated in elongated tracks that overlapped and extended well beyond the site of transcription. Splicing appears to occur directly within this RNA track, as evidenced by an unambiguous spatial separation of intron-containing and spliced transcripts. Excised introns for neurotensin RNA appear free to diffuse. The transcription and processing site of the fibronectin gene localized to the nuclear interior and was associated with larger transcript domains in over 88 percent of the cells. These results support a view of nuclear function closely integrated with structure.


Science. 1993 Feb 26;259(5099):1326-30.

Journal/Book/Conference Title

Science (New York, N.Y.)

Related Resources

Link to Article in PubMed

PubMed ID