GSBS Student Publications


Analysis of low zone tolerance induction in normal and B cell-deficient mice

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Pathology



Document Type


Medical Subject Headings

Animals; Antibody Formation; Antigen-Presenting Cells; B-Lymphocytes; Dose-Response Relationship, Immunologic; Female; Immune Tolerance; Immunologic Deficiency Syndromes; Injections, Intravenous; Lymphocyte Activation; Mice; Mice, Inbred C57BL; Mice, Mutant Strains; Mice, Transgenic; Ovalbumin; Self Tolerance; Solubility; Th1 Cells


Life Sciences | Medicine and Health Sciences


To investigate the role of B cells as APCs in acquired tolerance induced by low dose soluble protein Ags, normal and B cell-deficient adult mice were injected i.v. with repeated low doses (10 microgram) of deaggregated OVA, then challenged with OVA in CFA. In animals treated with deaggregated OVA, the in vitro proliferative responses of lymph node T cells to OVA were significantly reduced, and production of the Th1 cytokine, IFN-gamma, in response to OVA was reduced to undetectable levels. This occurred in both normal and B cell-deficient treated animals. B cells were also unnecessary for self tolerance of T cells to the transgenic self Ag, hen egg lysozyme, in a strain with a very low serum lysozyme concentration. Partial low zone tolerance induced by deaggregated, low dose OVA was selective for T cell responses as measured by in vitro proliferation and IL-2 and IFN-gamma production, because Ab responses of B cell-sufficient mice to this T cell-dependent Ag were largely unaffected. Both treated and untreated animals produced equivalent titers of anti-OVA Abs, predominantly of the IgG1 and IgG2b isotypes, following challenge with OVA in CFA.

Rights and Permissions

Citation: J Immunol. 1996 Sep 1;157(5):1833-9.

Related Resources

Link to article in PubMed

Journal Title

Journal of immunology (Baltimore, Md. : 1950)

PubMed ID