GSBS Student Publications
Title
Activation of the bone-related Runx2/Cbfa1 promoter in mesenchymal condensations and developing chondrocytes of the axial skeleton
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Cell Biology
Date
8-15-2002
Document Type
Article
Medical Subject Headings
Animals; Bone and Bones; Chondrocytes; Core Binding Factor Alpha 1 Subunit; Mesoderm; Mice; Mice, Transgenic; Models, Genetic; *Neoplasm Proteins; *Promoter Regions (Genetics); Protein Isoforms; Signal Transduction; Time Factors; Transcription Factors
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The Runx2/Cbfa1 transcription factor regulates a program of gene expression necessary for skeletal development. To understand signals mediating skeletal formation, we examined the in vivo spatio-temporal activity of the Runx2 P1 promoter which controls expression of the bone-related Type II isoform. Transgenic mice carrying 3 kb of Runx2 promoter fused to the lacZ gene exhibit localized promoter activity in early mesenchymal condensations shortly after the embryonic turning event. Expression in developing mesenchyme continues throughout chondrogenesis and is restricted to the axial skeleton. Our data support a function for Runx2 in establishment of the prechondrocytic skeletal primordium.
Rights and Permissions
Citation: Mech Dev. 2002 Jun;114(1-2):167-70.
Related Resources
Journal Title
Mechanisms of development
PubMed ID
12175505
Repository Citation
Lengner, Christopher J.; Drissi, Hicham; Choi, Je-Yong; Van Wijnen, Andre J.; Stein, Janet L.; Stein, Gary S.; and Lian, Jane B., "Activation of the bone-related Runx2/Cbfa1 promoter in mesenchymal condensations and developing chondrocytes of the axial skeleton" (2002). GSBS Student Publications. 671.
https://escholarship.umassmed.edu/gsbs_sp/671