GSBS Student Publications

Title

Genetic mosaic with dual binary transcriptional systems in Drosophila

Student Author(s)

Sen-Lin Lai

GSBS Program

Neuroscience

Publication Date

2006-5

UMMS Affiliation

Department of Neurobiology; Tzumin Lee Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Document Type

Article

Disciplines

Neuroscience and Neurobiology

Abstract

MARCM (mosaic analysis with a repressible cell marker) involves specific labeling of GAL80-minus and GAL4-positive homozygous cells in otherwise heterozygous tissues. Here we demonstrate how the concurrent use of two independent binary transcriptional systems may facilitate complex MARCM studies in the Drosophila nervous system. By fusing LexA with the VP16 acidic activation domain (VP16) or the GAL4 activation domain (GAD), we obtained both GAL80-insensitive and GAL80-suppressible transcriptional factors. LexA::VP16 can mediate MARCM-independent binary transgene induction in mosaic organisms. The incorporation of LexA::GAD into MARCM, which we call dual-expression-control MARCM, permits the induction of distinct transgenes in different patterns among GAL80-minus cells in mosaic tissues. Lineage analysis with dual-expression-control MARCM suggested the presence of neuroglioblasts in the developing optic lobes but did not indicate the production of glia by postembryonic mushroom body neuronal precursors. In addition, dual-expression-control MARCM with a ubiquitous LexA::GAD driver revealed many unidentified cells in the GAL4-GH146-positive projection neuron lineages.

DOI of Published Version

10.1038/nn1681

Source

Nat Neurosci. 2006 May;9(5):703-9. Epub 2006 Apr 2. Link to article on publisher's site

Journal/Book/Conference Title

Nature neuroscience

Related Resources

Link to article in PubMed

PubMed ID

16582903

Share

COinS