UMass Chan Affiliations
Department of Molecular Genetics and MicrobiologyGraduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
1982-01-01Keywords
*Cloning, Molecular; DNA; DNA, Recombinant; Escherichia coli; Female; *Fetus; Humans; Plasmids; Poly A; Pregnancy; RNALife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
We have constructed libraries of recombinant DNA plasmids containing sequences complementary to polyadenylated RNA from a variety of human midtrimester fetal tissues. The bacterial colonies containing these plasmids have been grown and replicated on nitrocellulose filters in a manner that facilitates permanent storage, rapid screening, and transportability to other laboratories. We screened a portion of the library for the presence of repetitive sequences and found that approximately 20% of the clones contain repetitive sequences. We have also shown that some clones contain nonrepetitive sequences. Pools of recombinant cDNA-containing plasmids devoid of repetitive sequences have been constructed to permit the chromosomal localization of a variety of actively transcribed sequences. The construction of such large, tissue-specific clone banks should facilitate the direct isolation, mapping, and characterization of normal and abnormal human genes.Source
Cytogenet Cell Genet. 1982;34(3):193-203.
DOI
10.1159/000131807Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33978PubMed ID
6183056Related Resources
ae974a485f413a2113503eed53cd6c53
10.1159/000131807