GSBS Student Publications

Title

Construction of cloned libraries from RNA of human fetal tissues

GSBS Program

Biochemistry & Molecular Pharmacology

Publication Date

1982-01-01

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We have constructed libraries of recombinant DNA plasmids containing sequences complementary to polyadenylated RNA from a variety of human midtrimester fetal tissues. The bacterial colonies containing these plasmids have been grown and replicated on nitrocellulose filters in a manner that facilitates permanent storage, rapid screening, and transportability to other laboratories. We screened a portion of the library for the presence of repetitive sequences and found that approximately 20% of the clones contain repetitive sequences. We have also shown that some clones contain nonrepetitive sequences. Pools of recombinant cDNA-containing plasmids devoid of repetitive sequences have been constructed to permit the chromosomal localization of a variety of actively transcribed sequences. The construction of such large, tissue-specific clone banks should facilitate the direct isolation, mapping, and characterization of normal and abnormal human genes.

Source

Cytogenet Cell Genet. 1982;34(3):193-203.

Journal/Book/Conference Title

Cytogenetics and cell genetics

Related Resources

Link to article in PubMed

PubMed ID

6183056

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