The Tec family tyrosine kinases Itk and Rlk regulate the development of conventional CD8+ T cells
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Department of Pathology
Life Sciences | Medicine and Health Sciences
The Tec family tyrosine kinases, Itk and Rlk, are expressed in thymocytes and peripheral T cells and regulate thresholds of T cell receptor signaling. Yet little is known about the specific role of Itk- and Rlk-dependent signals in CD8(+) T cell maturation. We show here that Itk(-/-) and Rlk(-/-)Itk(-/-) mice were nearly devoid of conventional CD8(+) T cells and, instead, contained a large population of CD8(+) T cells that bear striking similarity to lineages of innate lymphocytes. Itk(-/-) and Rlk(-/-)Itk(-/-) CD8(+) thymocytes and T cells were CD44(hi), CD122(+), and NK1.1(+); were able to produce interferon-gamma directly ex vivo; and were dependent on interleukin-15. Itk(-/-) and Rlk(-/-)Itk(-/-) CD8(+) thymocytes expressed abundant transcripts for the T box transcription factor, eomesodermin, correlating with their phenotype and function. These data indicate a critical role for Itk and Rlk in conventional CD8(+) T cell development in the thymus.
DOI of Published Version
Immunity. 2006 Jul;25(1):79-91. Link to article on publisher's site
Atherly, Luana O.; Lucas, Julie Ann; Felices, Martin; Yin, Catherine C.; Reiner, Steven L.; and Berg, Leslie J., "The Tec family tyrosine kinases Itk and Rlk regulate the development of conventional CD8+ T cells" (2006). GSBS Student Publications. 63.