The Tec family tyrosine kinases Itk and Rlk regulate the development of conventional CD8+ T cells
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Department of Pathology
Life Sciences | Medicine and Health Sciences
The Tec family tyrosine kinases, Itk and Rlk, are expressed in thymocytes and peripheral T cells and regulate thresholds of T cell receptor signaling. Yet little is known about the specific role of Itk- and Rlk-dependent signals in CD8(+) T cell maturation. We show here that Itk(-/-) and Rlk(-/-)Itk(-/-) mice were nearly devoid of conventional CD8(+) T cells and, instead, contained a large population of CD8(+) T cells that bear striking similarity to lineages of innate lymphocytes. Itk(-/-) and Rlk(-/-)Itk(-/-) CD8(+) thymocytes and T cells were CD44(hi), CD122(+), and NK1.1(+); were able to produce interferon-gamma directly ex vivo; and were dependent on interleukin-15. Itk(-/-) and Rlk(-/-)Itk(-/-) CD8(+) thymocytes expressed abundant transcripts for the T box transcription factor, eomesodermin, correlating with their phenotype and function. These data indicate a critical role for Itk and Rlk in conventional CD8(+) T cell development in the thymus.
DOI of Published Version
Immunity. 2006 Jul;25(1):79-91. Link to article on publisher's site
Atherly LO, Lucas JA, Felices M, Yin CC, Reiner SL, Berg LJ. (2006). The Tec family tyrosine kinases Itk and Rlk regulate the development of conventional CD8+ T cells. GSBS Student Publications. https://doi.org/10.1016/j.immuni.2006.05.012. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/63