GSBS Student Publications
Title
Cdc2 tyrosine phosphorylation is not required for the S-phase DNA damage checkpoint in fission yeast
GSBS Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Date
11-15-2006
Document Type
Article
Medical Subject Headings
CDC2 Protein Kinase; Cell Cycle; Cell Cycle Proteins; Cell Nucleus; *DNA Damage; Flow Cytometry; *Gene Expression Regulation, Fungal; Genotype; Phosphorylation; *S Phase; Schizosaccharomyces; Time Factors; Tyrosine
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The S-phase DNA damage checkpoint slows replication when damage occurs during S phase. Cdc25, which activates Cdc2 by dephosphorylating tyrosine-15, has been shown to be a downstream target of the checkpoint in metazoans, but its role is not clear in fission yeast. The dephosphorylation of Cdc2 has been assumed not to play a role in S-phase regulation because cells replicate in the absence of Cdc25, demonstrating that tyrosine-15 phosphorylated dc2 is sufficient for S phase. However, it has been reported recently that Cdc25 is involved in the slowing of S phase in response to damage in fission yeast, suggesting a modulatory role for Cdc2 dephosphorylation in S phase. We have investigated the role of Cdc25 and the tyrosine phosphorylation of Cdc2 in the S-phase damage checkpoint, and our results show that Cdc2 phosphorylation is not a target of the checkpoint. The checkpoint was not compromised in a Cdc25 overexpressing strain, a strain carrying nonphosphorylatable form of Cdc2, or in a strain lacking Cdc25. Our results are consistent with a strictly Cdc2-Y15 phosphorylation-independent mechanism of the fission yeast S-phase DNA damage checkpoint.
Rights and Permissions
Citation: Cell Cycle. 2006 Nov 1;5(21):2495-500. Epub 2006 Sep 19.
Related Resources
Journal Title
Cell cycle (Georgetown, Tex.)
PubMed ID
17102632
Repository Citation
Kommajosyula, Naveen and Rhind, Nicholas R., "Cdc2 tyrosine phosphorylation is not required for the S-phase DNA damage checkpoint in fission yeast" (2006). GSBS Student Publications. 620.
https://escholarship.umassmed.edu/gsbs_sp/620