GSBS Student Publications
Title
PGRP-LC and PGRP-LE have essential yet distinct functions in the drosophila immune response to monomeric DAP-type peptidoglycan
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Medicine, Division of Infectious Disease and Immunology
Date
6-13-2006
Document Type
Article
Medical Subject Headings
Amino Acid Motifs; Amino Acid Sequence; Animals; Bordetella pertussis; Carrier Proteins; Cell Membrane; Cells, Cultured; Diaminopimelic Acid; Drosophila Proteins; Drosophila melanogaster; Escherichia coli; Gene Expression Regulation; Hemolymph; Intracellular Fluid; Malpighian Tubules; Molecular Sequence Data; Peptide Fragments; Peptidoglycan; RNA Interference; Recombinant Fusion Proteins; Signal Transduction; Transfection; Virulence Factors, Bordetella
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Drosophila rely entirely on an innate immune response to combat microbial infection. Diaminopimelic acid-containing peptidoglycan, produced by Gram-negative bacteria, is recognized by two receptors, PGRP-LC and PGRP-LE, and activates a homolog of transcription factor NF-kappaB through the Imd signaling pathway. Here we show that full-length PGRP-LE acted as an intracellular receptor for monomeric peptidoglycan, whereas a version of PGRP-LE containing only the PGRP domain functioned extracellularly, like the mammalian CD14 molecule, to enhance PGRP-LC-mediated peptidoglycan recognition on the cell surface. Interaction with the imd signaling protein was not required for PGRP-LC signaling. Instead, PGRP-LC and PGRP-LE signaled through a receptor-interacting protein homotypic interaction motif-like motif. These data demonstrate that like mammals, drosophila use both extracellular and intracellular receptors, which have conserved signaling mechanisms, for innate immune recognition.
Rights and Permissions
Citation: Nat Immunol. 2006 Jul;7(7):715-23. Epub 2006 Jun 11. Link to article on publisher's site
DOI of Published Version
10.1038/ni1356
Related Resources
Journal Title
Nature immunology
PubMed ID
16767093
Repository Citation
Kaneko, Takashi; Yano, Tamaki; Aggarwal, Kamna; Lim, Jae-Hong; Ueda, Kazunori; Oshima, Yoshiteru; Peach, Camilla; Erturk Hasdemir, Deniz; Goldman, William E.; Oh, Byung-Ha; Kurata, Shoichiro; and Silverman, Neal S., "PGRP-LC and PGRP-LE have essential yet distinct functions in the drosophila immune response to monomeric DAP-type peptidoglycan" (2006). GSBS Student Publications. 590.
https://escholarship.umassmed.edu/gsbs_sp/590