GSBS Student Publications
Title
DOC-2/DAB2 is the binding partner of myosin VI
GSBS Program
Biochemistry & Molecular Pharmacology
Publication Date
2002-03-22
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Physiology
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Myosin VI is a molecular motor that moves processively along actin filaments and is believed to play a role in cargo movement in cells. Here we found that DOC-2/DAB2, a signaling molecule inhibiting the Ras cascade, binds to myosin VI at the globular tail domain. DOC-2/DAB2 binds stoichiometrically to myosin VI with one molecule per one myosin VI heavy chain. The C-terminal 122 amino acid residues of DOC-2/DAB2, containing the Grb2 binding site, is identified to be critical for the binding to myosin VI. Actin gliding assay revealed that the binding of DOC-2/DAB2 to myosin VI can support the actin filament gliding by myosin VI, suggesting that it can function as a myosin VI anchoring molecule. The C-terminal domain but not the N-terminal domain of DOC-2/DAB2 functions as a myosin VI anchoring site. The present findings suggest that myosin VI plays a role in transporting DOC-2/DAB2, a Ras cascade signaling molecule, thus involved in Ras signaling pathways.
DOI of Published Version
10.1006/bbrc.2002.6636
Source
Biochem Biophys Res Commun. 2002 Mar 29;292(2):300-7. Link to article on publisher's site
Journal/Book/Conference Title
Biochemical and biophysical research communications
Related Resources
PubMed ID
11906161
Repository Citation
Inoue, Akira; Sato, Osamu; Homma, Kazuaki; and Ikebe, Mitsuo, "DOC-2/DAB2 is the binding partner of myosin VI" (2002). GSBS Student Publications. 560.
https://escholarship.umassmed.edu/gsbs_sp/560