Department of Cell Biology
Cell Biology | Life Sciences | Medicine and Health Sciences
How centrosome removal or perturbations of centrosomal proteins leads to G1 arrest in untransformed mammalian cells has been a mystery. We use microsurgery and laser ablation to remove the centrosome from two types of normal human cells. First, we find that the cells assemble centrioles de novo after centrosome removal; thus, this phenomenon is not restricted to transformed cells. Second, normal cells can progress through G1 in its entirety without centrioles. Therefore, the centrosome is not a necessary, integral part of the mechanisms that drive the cell cycle through G1 into S phase. Third, we provide evidence that centrosome loss is, functionally, a stress that can act additively with other stresses to arrest cells in G1 in a p38-dependent fashion.
DOI of Published Version
J Cell Biol. 2007 Jan 15;176(2):173-82. Link to article on publisher's site
The Journal of cell biology
Uetake Y, Loncarek J, Nordberg JJ, English CN, La Terra S, Khodjakov A, Sluder G. (2007). Cell cycle progression and de novo centriole assembly after centrosomal removal in untransformed human cells. GSBS Student Publications. https://doi.org/10.1083/jcb.200607073. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/514