Yeast TAF(II)90 is required for cell-cycle progression through G2/M but not for general transcription activation
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Program in Molecular Medicine; Program in Gene Function and Expression
Life Sciences | Medicine and Health Sciences
The RNA polymerase II general transcription factor TFIID is a multisubunit complex comprising TATA-box binding protein and associated factors (TAFIIs). In vitro experiments have suggested that TAFIIs are essential coactivators required for RNA polymerase II-directed transcription activation. Here, for the first time, we analyze systematically the in vivo function of a specific TAFII, yeast TAFII90 (yTAFII90). We show that functional inactivation of yTAFII90 by temperature-sensitive mutations or depletion leads to arrest at the G2/M phase of the cell cycle. Unexpectedly, in the absence of functional yTAFII90, a variety of endogenous yeast genes were all transcribed normally, including those driven by well-characterized activators. Taken together, our results indicate that yTAFII90 is not required for transcription activation in general, and reveal linkages between TAF function and cell-cycle progression.
DOI of Published Version
Genes Dev. 1996 Sep 15;10(18):2368-80.
Genes and development
Apone LM, Virbasius CA, Reese JC, Green MR. (1996). Yeast TAF(II)90 is required for cell-cycle progression through G2/M but not for general transcription activation. GSBS Student Publications. https://doi.org/10.1101/gad.10.18.2368. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/50