GSBS Student Publications
Title
Human peripheral blood xenografts in the SCID mouse: characterization of immunologic reconstitution
GSBS Program
Biochemistry & Molecular Pharmacology
Publication Date
1993-09-01
UMMS Affiliation
Graduate School of Biomedical Sciences; Program in Immunology and Virology; Department of Pediatrics
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Immune reconstitutions (hu-PBL-SCID mice) resulting from adoptive transfer of human peripheral blood mononuclear cells into 1800 C.B-17 scid-/scid-mice were characterized. Over 90% of reconstitutions were successful as evidenced by human immunoglobulin production. Variability was noted with donor, cell number, and cell type. Human cells (T lymphocytes, few B cells) could be recovered by 5 days after engraftment. High levels of soluble CD8 and interleukin-2 receptors were detected in sera of hu-PBL-SCID mice. Cells recovered from 17 mice proliferated in response to antigens to which the donor had been primed; responses to nonboosted antigen also increased in some animals. After reconstitution, lymphocytes were found in the spleen and lymph nodes without full restoration of normal architecture. The hu-PBL-SCID mouse shows promise as a model system for a variety of immunologic studies. The inherent variation in the system must be minimized for appropriate use of the model.
DOI of Published Version
10.1093/infdis/168.3.630
Source
J Infect Dis. 1993 Sep;168(3):630-40.
Journal/Book/Conference Title
The Journal of infectious diseases
Related Resources
PubMed ID
8354904
Repository Citation
Hesselton, RuthAnn M.; Koup, Richard A.; Cromwell, Mary A.; Graham, Barney S.; Johns, Michael; and Sullivan, John L., "Human peripheral blood xenografts in the SCID mouse: characterization of immunologic reconstitution" (1993). GSBS Student Publications. 493.
https://escholarship.umassmed.edu/gsbs_sp/493