Title
Human peripheral blood xenografts in the SCID mouse: characterization of immunologic reconstitution
UMMS Affiliation
Graduate School of Biomedical Sciences; Program in Immunology and Virology; Department of Pediatrics
Publication Date
1993-09-01
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Immune reconstitutions (hu-PBL-SCID mice) resulting from adoptive transfer of human peripheral blood mononuclear cells into 1800 C.B-17 scid-/scid-mice were characterized. Over 90% of reconstitutions were successful as evidenced by human immunoglobulin production. Variability was noted with donor, cell number, and cell type. Human cells (T lymphocytes, few B cells) could be recovered by 5 days after engraftment. High levels of soluble CD8 and interleukin-2 receptors were detected in sera of hu-PBL-SCID mice. Cells recovered from 17 mice proliferated in response to antigens to which the donor had been primed; responses to nonboosted antigen also increased in some animals. After reconstitution, lymphocytes were found in the spleen and lymph nodes without full restoration of normal architecture. The hu-PBL-SCID mouse shows promise as a model system for a variety of immunologic studies. The inherent variation in the system must be minimized for appropriate use of the model.
DOI of Published Version
10.1093/infdis/168.3.630
Source
J Infect Dis. 1993 Sep;168(3):630-40.
Journal/Book/Conference Title
The Journal of infectious diseases
Related Resources
PubMed ID
8354904
Repository Citation
Hesselton RM, Koup RA, Cromwell MA, Graham BS, Johns M, Sullivan JL. (1993). Human peripheral blood xenografts in the SCID mouse: characterization of immunologic reconstitution. GSBS Student Publications. https://doi.org/10.1093/infdis/168.3.630. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/493