Title
Transcription factors RUNX1/AML1 and RUNX2/Cbfa1 dynamically associate with stationary subnuclear domains
UMMS Affiliation
Department of Cell Biology and Cancer Center; Department of Physiology
Publication Date
2002-10-03
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The runt-related transcription factors (RUNX/Cbfa/AML) are essential for cellular differentiation and fetal development. C-terminal truncations of RUNX factors that eliminate the targeting of these factors to subnuclear foci result in lethal hematopoietic and skeletal phenotypes. Here we demonstrate that in living cells the RUNX C-terminus is necessary for the dynamic association of RUNX into stable subnuclear domains. Time-lapse fluorescence microscopy shows that RUNX1 and RUNX2 localize to punctate foci that remain stationary in the nuclear space. By fluorescence recovery after photobleaching assays, both proteins are shown to dynamically associate at these subnuclear foci, with a 10 second half-time of recovery. A truncation of RUNX2, removing its intranuclear targeting signal (NMTS), increases its mobility by an order of magnitude, resulting in a half-time of recovery equivalent to that of EGFP alone. We propose that the dynamic shuttling of RUNX factors in living cells to positionally stabilized foci, which is dependent on the C-terminus, is a component of the mechanism for gene regulation in vivo.
DOI of Published Version
10.1242/jcs.00095
Source
J Cell Sci. 2002 Nov 1;115(Pt 21):4167-76.
Journal/Book/Conference Title
Journal of cell science
Related Resources
PubMed ID
12356919
Repository Citation
Harrington KS, Javed A, Drissi H, McNeil SM, Lian JB, Stein JL, Van Wijnen AJ, Wang Y, Stein GS. (2002). Transcription factors RUNX1/AML1 and RUNX2/Cbfa1 dynamically associate with stationary subnuclear domains. GSBS Student Publications. https://doi.org/10.1242/jcs.00095. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/473