Title
Interaction and specificity of Rel-related proteins in regulating Drosophila immunity gene expression
UMMS Affiliation
Program in Molecular Medicine; Department of Biochemistry and Molecular Pharmacology
Publication Date
1999-07-20
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
NF-kappaB/Rel family proteins regulate genes that are critical for many cellular processes including apoptosis, inflammation, immune response, and development. NF-kappaB/Rel proteins function as homodimers or heterodimers, which recognize specific DNA sequences within target promoters. We examined the activity of different Drosophila Rel-related proteins in modulating Drosophila immunity genes by expressing the Rel proteins in stably transfected cell lines. We also compared how different combinations of these transcriptional regulators control the activity of various immunity genes. The results show that Rel proteins are directly involved in regulating the Drosophila antimicrobial response. Furthermore, the drosomycin and defensin expression is best induced by the Relish/Dif and the Relish/Dorsal heterodimers, respectively, whereas the attacin activity can be efficiently up-regulated by the Relish homodimer and heterodimers. These results illustrate how the formation of Rel protein dimers differentially regulate target gene expression.
Source
J Biol Chem. 1999 Jul 23;274(30):21355-61.
Journal/Book/Conference Title
The Journal of biological chemistry
Related Resources
PubMed ID
10409696
Repository Citation
Han Z, Ip YT. (1999). Interaction and specificity of Rel-related proteins in regulating Drosophila immunity gene expression. GSBS Student Publications. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/469