GSBS Student Publications
Title
Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62
GSBS Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Graduate School of Biomedical Sciences; Program in Molecular Medicine
Date
4-15-1993
Document Type
Article
Medical Subject Headings
Amino Acid Sequence; Animals; Base Sequence; Calcium-Calmodulin-Dependent Protein Kinases; Cell Line; Cercopithecus aethiops; Codon; DNA; Gene Expression; Genes, myc; Humans; Molecular Sequence Data; *Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Phosphorylation; Plasmids; Point Mutation; Polymerase Chain Reaction; Protein Kinases; Proto-Oncogene Proteins c-myc; *Serine; Substrate Specificity; *Threonine; *Trans-Activation (Genetics)
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The product of the human c-myc protooncogene (Myc) is a sequence-specific DNA binding protein. Here, we demonstrate that the placement of the specific Myc DNA binding site CACGTG upstream of a luciferase reporter gene conferred Myc-stimulated expression that was inhibited by the overexpression of the basic-helix-loop-helix/leucine zipper protein Max. It was observed that Myc was phosphorylated in vivo within the NH2-terminal domain at Thr-58 and Ser-62. Replacement of these phosphorylation sites with Ala residues caused a marked decrease in Myc-stimulated reporter gene expression. In contrast, the replacement of Thr-58 or Ser-62 with an acidic residue (Glu) caused only a small inhibition of transactivation. Together, these data demonstrate that the NH2-terminal phosphorylation sites Thr-58 and Ser-62 are required for high levels of transactivation of gene expression by Myc.
Rights and Permissions
Citation: Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3216-20.
Related Resources
Journal Title
Proceedings of the National Academy of Sciences of the United States of America
PubMed ID
8386367
Repository Citation
Gupta, Shashi; Seth, Alpna; and Davis, Roger J., "Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62" (1993). GSBS Student Publications. 460.
https://escholarship.umassmed.edu/gsbs_sp/460