Aberrant termination triggers nonsense-mediated mRNA decay
Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology
Life Sciences | Medicine and Health Sciences
NMD (nonsense-mediated mRNA decay) is a cellular quality-control mechanism in which an otherwise stable mRNA is destabilized by the presence of a premature termination codon. We have defined the set of endogenous NMD substrates, demonstrated that they are available for NMD at every round of translation, and showed that premature termination and normal termination are not equivalent biochemical events. Premature termination is aberrant, and its NMD-stimulating defects can be reversed by the presence of tethered poly(A)-binding protein (Pab1p) or tethered eRF3 (eukaryotic release factor 3) (Sup35p). Thus NMD appears to be triggered by a ribosome's failure to terminate adjacent to a properly configured 3'-UTR (untranslated region), an event that may promote binding of the UPF/NMD factors to stimulate mRNA decapping.
DOI of Published Version
Biochem Soc Trans. 2006 Feb;34(Pt 1):39-42. Link to article on publisher's site
Biochemical Society transactions
Amrani N, Dong S, He F, Ganesan R, Ghosh S, Kervestin S, Li C, Mangus DA, Spatrick P, Jacobson A. (2005). Aberrant termination triggers nonsense-mediated mRNA decay. GSBS Student Publications. https://doi.org/10.1042/BST20060039. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/46