The nuclear matrix protein NMP-1 is the transcription factor YY1
Department of Cell Biology
Cell Biology | Life Sciences | Medicine and Health Sciences
NMP-1 was initially identified as a nuclear matrix-associated DNA-binding factor that exhibits sequence-specific recognition for the site IV regulatory element of a histone H4 gene. This distal promoter domain is a nuclear matrix interaction site. In the present study, we show that NMP-1 is the multifunctional transcription factor YY1. Gel-shift and Western blot analyses demonstrate that NMP-1 is immunoreactive with YY1 antibody. Furthermore, purified YY1 protein specifically recognizes site IV and reconstitutes the NMP-1 complex. Western blot and gel-shift analyses indicate that YY1 is present within the nuclear matrix. In situ immunofluorescence studies show that a significant fraction of YY1 is localized in the nuclear matrix, principally but not exclusively associated with residual nucleoli. Our results confirm that NMP-1/YY1 is a ubiquitous protein that is present in both human cells and in rat osteosarcoma ROS 17/2.8 cells. The finding that NMP-1 is identical to YY1 suggests that this transcriptional regulator may mediate gene-matrix interactions. Our results are consistent with the concept that the nuclear matrix may functionally compartmentalize the eukaryotic nucleus to support regulation of gene expression.
DOI of Published Version
Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10526-30.
Proceedings of the National Academy of Sciences of the United States of America
Guo B, Odgren PR, Van Wijnen AJ, Last TJ, Nickerson JA, Penman S, Lian JB, Stein JL, Stein GS. (1995). The nuclear matrix protein NMP-1 is the transcription factor YY1. GSBS Student Publications. https://doi.org/10.1073/pnas.92.23.10526. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/457