GSBS Student Publications
Title
Cortical actin turnover during cytokinesis requires myosin II
GSBS Program
Cancer Biology
UMMS Affiliation
Department of Physiology
Date
4-28-2005
Document Type
Article
Medical Subject Headings
Actins; Animals; Calcium-Calmodulin-Dependent Protein Kinases; Cells, Cultured; Cytokinesis; Fluorescence Recovery After Photobleaching; Heterocyclic Compounds with 4 or More Rings; Myosin Type II; Protein Transport; Protozoan Proteins; Quinolinium Compounds; Rats
Disciplines
Cell and Developmental Biology | Life Sciences | Medicine and Health Sciences
Abstract
The involvement of myosin II in cytokinesis has been demonstrated with microinjection, genetic, and pharmacological approaches; however, the exact role of myosin II in cell division remains poorly understood. To address this question, we treated dividing normal rat kidney (NRK) cells with blebbistatin, a potent inhibitor of the nonmuscle myosin II ATPase. Blebbistatin caused a strong inhibition of cytokinesis but no detectable effect on the equatorial localization of actin or myosin. However, whereas these filaments dissociated from the equator in control cells during late cytokinesis, they persisted in blebbistatin-treated cells over an extended period of time. The accumulation of equatorial actin was caused by the inhibition of actin filament turnover, as suggested by a 2-fold increase in recovery half-time after fluorescence photobleaching. Local release of blebbistatin at the equator caused localized accumulation of equatorial actin and inhibition of cytokinesis, consistent with the function of myosin II along the furrow. However, treatment of the polar region also caused a high frequency of abnormal cytokinesis, suggesting that myosin II may play a second, global role. Our observations indicate that myosin II ATPase is not required for the assembly of equatorial cortex during cytokinesis but is essential for its subsequent turnover and remodeling.
Rights and Permissions
Citation: Curr Biol. 2005 Apr 26;15(8):732-6. Link to article on publisher's site
DOI of Published Version
10.1016/j.cub.2005.03.042
Related Resources
Journal Title
Current biology : CB
PubMed ID
15854905
Repository Citation
Guha, Minakshi; Zhou, Mian; and Wang, Yu-Li, "Cortical actin turnover during cytokinesis requires myosin II" (2005). GSBS Student Publications. 453.
https://escholarship.umassmed.edu/gsbs_sp/453