Title
A predominantly nuclear protein affecting cytoplasmic localization of beta-actin mRNA in fibroblasts and neurons
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Cell Biology
Publication Date
2002-01-10
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The localization of beta-actin mRNA to the leading lamellae of chicken fibroblasts and neurite growth cones of developing neurons requires a 54-nt localization signal (the zipcode) within the 3' untranslated region. In this study we have identified and isolated five proteins binding to the zipcode. One of these we previously identified as zipcode binding protein (ZBP)1, a 4-KH domain protein. A second is now investigated in detail: a 92-kD protein, ZBP2, that is especially abundant in extracts from embryonic brain. We show that ZBP2 is a homologue of the human hnRNP protein, KSRP, that appears to mediate pre-mRNA splicing. However, ZBP2 has a 47-amino acid (aa) sequence not present in KSRP. Various portions of ZBP2 fused to GFP indicate that the protein most likely shuttles between the nucleus and the cytoplasm, and that the 47-aa insert promotes the nuclear localization. Expression of a truncated ZBP2 inhibits the localization of beta-actin mRNA in both fibroblast and neurons. These data suggest that ZBP2, although predominantly a nuclear protein, has a role in the cytoplasmic localization of beta-actin mRNA.
DOI of Published Version
10.1083/jcb.200105133
Source
J Cell Biol. 2002 Jan 7;156(1):41-51. Epub 2002 Jan 7. Link to article on publisher's site
Journal/Book/Conference Title
The Journal of cell biology
Related Resources
PubMed ID
11781334
Repository Citation
Gu W, Pan F, Zhang H, Bassell GJ, Singer RH. (2002). A predominantly nuclear protein affecting cytoplasmic localization of beta-actin mRNA in fibroblasts and neurons. GSBS Student Publications. https://doi.org/10.1083/jcb.200105133. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/447