Title
Genetic requirements for inheritance of RNAi in C. elegans
UMMS Affiliation
Graduate School of Biomedical Sciences; Program in Molecular Medicine; Department of Cell Biology
Publication Date
2000-03-31
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
In Caenorhabditis elegans, the introduction of double-stranded RNA triggers sequence-specific genetic interference (RNAi) that is transmitted to offspring. The inheritance properties associated with this phenomenon were examined. Transmission of the interference effect occurred through a dominant extragenic agent. The wild-type activities of the RNAi pathway genes rde-1 and rde-4 were required for the formation of this interfering agent but were not needed for interference thereafter. In contrast, the rde-2 and mut-7 genes were required downstream for interference. These findings provide evidence for germ line transmission of an extragenic sequence-specific silencing factor and implicate rde-1 and rde-4 in the formation of the inherited agent.
DOI of Published Version
10.1126/science.287.5462.2494
Source
Science. 2000 Mar 31;287(5462):2494-7.
Journal/Book/Conference Title
Science (New York, N.Y.)
Related Resources
PubMed ID
10741970
Repository Citation
Grishok A, Tabara H, Mello CC. (2000). Genetic requirements for inheritance of RNAi in C. elegans. GSBS Student Publications. https://doi.org/10.1126/science.287.5462.2494. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/417