GSBS Student Publications
Title
Genetic requirements for inheritance of RNAi in C. elegans
GSBS Program
Biochemistry & Molecular Pharmacology
Publication Date
2000-03-31
UMMS Affiliation
Graduate School of Biomedical Sciences; Program in Molecular Medicine; Department of Cell Biology
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
In Caenorhabditis elegans, the introduction of double-stranded RNA triggers sequence-specific genetic interference (RNAi) that is transmitted to offspring. The inheritance properties associated with this phenomenon were examined. Transmission of the interference effect occurred through a dominant extragenic agent. The wild-type activities of the RNAi pathway genes rde-1 and rde-4 were required for the formation of this interfering agent but were not needed for interference thereafter. In contrast, the rde-2 and mut-7 genes were required downstream for interference. These findings provide evidence for germ line transmission of an extragenic sequence-specific silencing factor and implicate rde-1 and rde-4 in the formation of the inherited agent.
DOI of Published Version
10.1126/science.287.5462.2494
Source
Science. 2000 Mar 31;287(5462):2494-7.
Journal/Book/Conference Title
Science (New York, N.Y.)
Related Resources
PubMed ID
10741970
Repository Citation
Grishok, Alla; Tabara, Hiroaki; and Mello, Craig C., "Genetic requirements for inheritance of RNAi in C. elegans" (2000). GSBS Student Publications. 417.
https://escholarship.umassmed.edu/gsbs_sp/417