GSBS Student Publications
Title
Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing
GSBS Program
Biochemistry & Molecular Pharmacology
Publication Date
2001-07-20
UMMS Affiliation
Graduate School of Biomedical Sciences; Program in Molecular Medicine; Department of Cell Biology
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
RNAi is a gene-silencing phenomenon triggered by double-stranded (ds) RNA and involves the generation of 21 to 26 nt RNA segments that guide mRNA destruction. In Caenorhabditis elegans, lin-4 and let-7 encode small temporal RNAs (stRNAs) of 22 nt that regulate stage-specific development. Here we show that inactivation of genes related to RNAi pathway genes, a homolog of Drosophila Dicer (dcr-1), and two homologs of rde-1 (alg-1 and alg-2), cause heterochronic phenotypes similar to lin-4 and let-7 mutations. Further we show that dcr-1, alg-1, and alg-2 are necessary for the maturation and activity of the lin-4 and let-7 stRNAs. Our findings suggest that a common processing machinery generates guide RNAs that mediate both RNAi and endogenous gene regulation.
DOI of Published Version
10.1016/S0092-8674(01)00431-7
Source
Cell. 2001 Jul 13;106(1):23-34.
Journal/Book/Conference Title
Cell
Related Resources
PubMed ID
11461699
Repository Citation
Grishok, Alla; Pasquinelli, Amy E.; Conte, Darryl Jr.; Li, Na; Parrish, Susan; Ha, Ilho; Baillie, David L.; Fire, Andrew Z.; Ruvkun, Gary; and Mello, Craig C., "Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing" (2001). GSBS Student Publications. 416.
https://escholarship.umassmed.edu/gsbs_sp/416