GSBS Student Publications
Title
The effects of mutations in the ant promoter of phage P22 depend on context
GSBS Program
Biochemistry & Molecular Pharmacology
Publication Date
1988-10-01
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Recombination was used to construct 22 two- or three-way combinations of down- and up-mutations in Pant, a strong, near-consensus promoter of phage P22. The relative strengths of these promoters in vivo were assayed by fusing them to an ant/lacZ gene fusion and measuring beta-galactosidase levels produced by lysogens carrying the fusions on single-copy prophages. The results of these assays show that the magnitude of the effect of a promoter mutation can vary considerably when its context is changed by the presence of another mutation. In addition, as Pant approaches conformity with the consensus promoter sequence, the up-mutations decrease promoter strength, even though the same mutations increase promoter strength in the presence of a down-mutation. These context effects imply that individual consensus base pairs cannot be considered to contribute to promoter strength independently.
Source
Genetics. 1988 Oct;120(2):319-27.
Journal/Book/Conference Title
Genetics
Related Resources
PubMed ID
3143618
Repository Citation
Grana, Dolores; Gardella, Thomas James; and Susskind, Miriam M., "The effects of mutations in the ant promoter of phage P22 depend on context" (1988). GSBS Student Publications. 408.
https://escholarship.umassmed.edu/gsbs_sp/408