Effect of substrate mechanics on chondrocyte adhesion to modified alginate surfaces
Graduate School of Biomedical Sciences; Center for Tissue Engineering
Life Sciences | Medicine and Health Sciences
This study characterized the attachment of chondrocytes to RGD-functionalized alginate by examining the effect of substrate stiffness on cell attachment and morphology. Bovine chondrocytes were added to wells coated with 2% alginate or RGD-alginate. The alginate was crosslinked with divalent cations ranging from 1.25 to 62.5 mmol/g alginate. Attachment to RGD-alginate was 10-20 times higher than attachment to unmodified alginate and was significantly inhibited by antibodies to integrin subunits alpha3l and beta1, cytochalasin-D, and soluble RGD peptide. The equilibrium level and rate of attachment increased with crosslink density and substrate stiffness. Substrate stiffness also regulated chondrocyte morphology, which changed from a rounded shape with nebulous actin on weaker substrates to a predominantly flat morphology with actin stress fibers on stiffer substrates. The dependence of attachment on integrins and substrate stiffness suggests that chondrocyte integrins may play a role in sensing the mechanical properties of the matrices to which they are attached.
DOI of Published Version
Arch Biochem Biophys. 2004 Feb 15;422(2):161-7. Link to article on publisher's site
Archives of biochemistry and biophysics
Genes NG, Rowley JA, Mooney DJ, Bonassar LJ. (2004). Effect of substrate mechanics on chondrocyte adhesion to modified alginate surfaces. GSBS Student Publications. https://doi.org/10.1016/j.abb.2003.11.023. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/393