Fat-specific protein 27, a novel lipid droplet protein that enhances triglyceride storage
Graduate School of Biomedical Sciences; Program in Molecular Medicine
Life Sciences | Medicine and Health Sciences
Fat-specific protein (FSP)27/Cidec is most highly expressed in white and brown adipose tissues and increases in abundance by over 50-fold during adipogenesis. However, its function in adipocytes has remained elusive since its discovery over 15 years ago. Here we demonstrate that FSP27/Cidec localizes to lipid droplets in cultured adipocytes and functions to promote lipid accumulation. Ectopically expressed FSP27-GFP surrounds lipid droplets in 3T3-L1 adipocytes and colocalizes with the known lipid droplet protein perilipin. Immunostaining of endogenous FSP27 in 3T3-L1 adipocytes also confirmed its presence on lipid droplets. FSP27-GFP expression also markedly increases lipid droplet size and enhances accumulation of total neutral lipids in 3T3-L1 preadipocytes as well as other cell types such as COS cells. Conversely, RNA interference-based FSP27/Cidec depletion in mature adipocytes significantly stimulates lipolysis and reduces the size of lipid droplets. These data reveal FSP27/Cidec as a novel adipocyte lipid droplet protein that negatively regulates lipolysis and promotes triglyceride accumulation.
DOI of Published Version
J Biol Chem. 2007 Nov 23;282(47):34213-8. Epub 2007 Sep 19. Link to article on publisher's site
The Journal of biological chemistry
Puri V, Konda S, Ranjit S, Aouadi M, Chawla A, Chouinard MT, Chakladar A, Czech MP. (2007). Fat-specific protein 27, a novel lipid droplet protein that enhances triglyceride storage. GSBS Student Publications. https://doi.org/10.1074/jbc.M707404200. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/375