Title
Sequence-specific DNA-binding proteins are components of a nuclear matrix-attachment site
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Cell Biology
Publication Date
1992-05-01
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
We have identified a nuclear matrix-attachment region within an upstream element of a human H4 histone gene promoter. Nuclear matrix proteins, isolated and solubilized from HeLa S3 cells, were found to interact with sequence specificity at this matrix-attachment region. Several types of assays for protein-DNA interaction showed that the minimal sequence for the nuclear matrix protein-DNA interaction was 5'-TGACGTCCATG-3'; the underlined region corresponds to the core consensus sequence for ATF transcription factor binding. Two proteins with molecular masses of 43 and 54 kDa were identified by UV-crosslinking analysis as integral components of this protein-DNA complex. The molecular masses of these proteins and the ATF-binding site consensus sequence suggest that these proteins are members of the ATF family. Our results provide direct evidence for nuclear matrix localization of sequence-specific DNA-binding factors for an actively transcribed gene. The proximity of a strong positive transcriptional regulatory element to the matrix-attachment region of this gene suggests that the nuclear matrix may serve to localize and concentrate trans-acting factors that facilitate regulation of gene expression.
DOI of Published Version
10.1073/pnas.89.9.4178
Source
Proc Natl Acad Sci U S A. 1992 May 1;89(9):4178-82.
Journal/Book/Conference Title
Proceedings of the National Academy of Sciences of the United States of America
Related Resources
PubMed ID
1570345
Repository Citation
Dworetzky SI, Wright KL, Fey EG, Penman S, Lian JB, Stein JL, Stein GS. (1992). Sequence-specific DNA-binding proteins are components of a nuclear matrix-attachment site. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1073/pnas.89.9.4178. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/336