Sequence-specific DNA-binding proteins are components of a nuclear matrix-attachment site
Graduate School of Biomedical Sciences; Department of Cell Biology
Life Sciences | Medicine and Health Sciences
We have identified a nuclear matrix-attachment region within an upstream element of a human H4 histone gene promoter. Nuclear matrix proteins, isolated and solubilized from HeLa S3 cells, were found to interact with sequence specificity at this matrix-attachment region. Several types of assays for protein-DNA interaction showed that the minimal sequence for the nuclear matrix protein-DNA interaction was 5'-TGACGTCCATG-3'; the underlined region corresponds to the core consensus sequence for ATF transcription factor binding. Two proteins with molecular masses of 43 and 54 kDa were identified by UV-crosslinking analysis as integral components of this protein-DNA complex. The molecular masses of these proteins and the ATF-binding site consensus sequence suggest that these proteins are members of the ATF family. Our results provide direct evidence for nuclear matrix localization of sequence-specific DNA-binding factors for an actively transcribed gene. The proximity of a strong positive transcriptional regulatory element to the matrix-attachment region of this gene suggests that the nuclear matrix may serve to localize and concentrate trans-acting factors that facilitate regulation of gene expression.
DOI of Published Version
Proc Natl Acad Sci U S A. 1992 May 1;89(9):4178-82.
Proceedings of the National Academy of Sciences of the United States of America
Dworetzky SI, Wright KL, Fey EG, Penman S, Lian JB, Stein JL, Stein GS. (1992). Sequence-specific DNA-binding proteins are components of a nuclear matrix-attachment site. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1073/pnas.89.9.4178. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/336