Title
Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways
UMMS Affiliation
Graduate School of Biomedical Sciences; Program in Molecular Medicine
Publication Date
2006-01-28
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
In plants, animals, and fungi, members of the Dicer family of RNase III-related enzymes process double-stranded RNA (dsRNA) to initiate small-RNA-mediated gene-silencing mechanisms. To learn how C. elegans Dicer, DCR-1, functions in multiple distinct silencing mechanisms, we used a mass-spectrometry-based proteomics approach to identify DCR-1-interacting proteins. We then generated and characterized deletion alleles for the corresponding genes. The interactors are required for production of three species of small RNA, including (1) small interfering RNAs (siRNAs), derived from exogenous dsRNA triggers (exo-siRNAs); (2) siRNAs derived from endogenous triggers (endo-siRNAs); and (3) developmental regulatory microRNAs (miRNAs). One interactor, the conserved RNA-phosphatase homolog PIR-1, is required for the processing of a putative amplified DCR-1 substrate. Interactors required for endo-siRNA production include ERI-1 and RRF-3, whose loss of function enhances RNAi. Our findings provide a first glimpse at the complex biochemical niche of Dicer and suggest that competition exists between DCR-1-mediated small-RNA pathways.
DOI of Published Version
10.1016/j.cell.2005.11.036
Source
Cell. 2006 Jan 27;124(2):343-54. Link to article on publisher's site
Journal/Book/Conference Title
Cell
Related Resources
PubMed ID
16439208
Repository Citation
Duchaine TF, Wohlschlegel JA, Kennedy S, Bei Y, Conte D, Pang KM, Brownell DR, Harding S, Mitani S, Ruvkun G, Yates JR, Mello CC. (2006). Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1016/j.cell.2005.11.036. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/330