UMMS Affiliation
Department of Pediatrics
Publication Date
2000-02-05
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences | Virus Diseases
Abstract
We analyzed the env genes of cytopathic and noncytopathic biological clones derived from two HIV-1-infected children with discordant clinical courses. Chimeric viruses were constructed by switching env regions from V2 through V3 of the biological clones with the corresponding region from the molecular clone NL4-3. These HIV-1 chimeric viruses exhibited similar replication kinetics as well as syncytium-inducing abilities. The chimeric virus containing the env region of noncytopathic biological clone, GC6 8-4, was noncytopathic in an in vitro cell-killing assay, while the chimeric virus containing the env region of cytopathic biological clone, HC4, was cytopathic in the in vitro cell-killing assay. These studies suggest the presence of a cytopathicity determinant that maps to the envelope sequences contained within the downstream region of V2 and within the V3 region (nucleotide position 6822 to nucleotide position 7250, based on NL4-3 sequence).
Rights and Permissions
This is a copy of an article published in AIDS Research and Human Retroviruses ©2000 Mary Ann Liebert, Inc.; AIDS Research and Human Retroviruses is available online at: http://www.liebertonline.com.
DOI of Published Version
10.1089/088922200309476
Source
AIDS Res Hum Retroviruses. 2000 Jan 20;16(2):125-37. Link to article on publisher's site
Journal/Book/Conference Title
AIDS research and human retroviruses
Related Resources
PubMed ID
10659052
Repository Citation
Forte SE, Somasundaran M, Sullivan JL. (2000). Attenuation of human immunodeficiency virus type 1 cytopathic effects by replacing a 424-bp region of envelope from a noncytopathic biological clone. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1089/088922200309476. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/302