Department of Pediatrics; Program in Molecular Medicine
Life Sciences | Medicine and Health Sciences | Virus Diseases
Human immunodeficiency virus type 1 (HIV-1) isolates from six infected individuals less then 4 years of age were phenotyped for their syncytium-inducing (SI) ability in MT-2 cells. Three viral isolates that induced syncytia were detected. One SI isolate was from an individual who was in disease stage P2A,B,C and two SI isolates were recovered sequentially from another individual who switched from disease stage P1B to P2F. Non-syncytium-inducing (NSI) isolates were detected in two individuals who were in stage P1B of disease, and in a third individual who was in stage P2A of disease. Three sequential isolates obtained over a 2-year period from a fourth individual who progressed from disease stage P1B to P2A,B,C and subsequently died of AIDS-related disease were also found to have the NSI phenotype. To test whether NSI isolates can replicate in the absence of syncytium formation, we analyzed NSI-infected MT-2 cells for production of viral p24 antigen and expression of viral RNA by in situ hybridization. By day 12 postinfection, 6 of 7 NSI viral isolates produced 7- to 36-fold increases in p24 antigen compared to day 6, and expressed viral RNA in 13-20% of cells. A single NSI isolate that did not replicate in MT-2 cells was obtained from an individual who was asymptomatic (stage P1B). The individual rapidly progressed to symptomatic stage P2F and two sequential SI viruses were isolated. These SI isolates replicated in MT-2 cells and induced cytopathic effects.(ABSTRACT TRUNCATED AT 250 WORDS)
Rights and Permissions
This is a copy of an article published in AIDS Research and Human Retroviruses © 1994 Mary Ann Liebert, Inc.; AIDS Research and Human Retroviruses is available online at: http://www.liebertonline.com.
DOI of Published Version
AIDS Res Hum Retroviruses. 1994 Dec;10(12):1613-8. doi:10.1089/aid.1994.10.1613. Link to article on publisher's website
AIDS research and human retroviruses
Forte, Serene Elizabeth; Byron, Kevin S.; Sullivan, John L.; and Somasundaran, Mohan, "Non-syncytium-inducing HIV type 1 isolated from infected individuals replicates in MT-2 cells" (1994). GSBS Student Publications. 301.