GSBS Student Publications

Title

A role for the lissencephaly gene LIS1 in mitosis and cytoplasmic dynein function

GSBS Program

Biochemistry & Molecular Pharmacology

Publication Date

2000-11-01

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Physiology; Department of Cell Biology

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Mutations in the LIS1 gene cause gross histological disorganization of the developing human brain, resulting in a brain surface that is almost smooth. Here we show that LIS1 protein co-immunoprecipitates with cytoplasmic dynein and dynactin, and localizes to the cell cortex and to mitotic kinetochores, which are known sites for binding of cytoplasmic dynein. Overexpression of LIS1 in cultured mammalian cells interferes with mitotic progression and leads to spindle misorientation. Injection of anti-LIS1 antibody interferes with attachment of chromosomes to the metaphase plate, and leads to chromosome loss. We conclude that LIS1 participates in a subset of dynein functions, and may regulate the division of neuronal progenitor cells in the developing brain.

DOI of Published Version

10.1038/35041020

Source

Nat Cell Biol. 2000 Nov;2(11):784-91. Link to article on publisher's site

Journal/Book/Conference Title

Nature cell biology

Related Resources

Link to article in PubMed

PubMed ID

11056532

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