Title
Tat stimulates cotranscriptional capping of HIV mRNA
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Publication Date
2002-11-01
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Here we investigated how capping and methylation of HIV pre-mRNAs are coupled to Pol II elongation. Stable binding of the capping enzyme (Mce1) and cap methyltransferase (Hcm1) to template-engaged Pol II depends on CTD phosphorylation, but not on nascent RNA. Both Mce1 and Hcm1 travel with Pol II during elongation. The capping and methylation reactions cannot occur until the nascent pre-mRNA has attained a chain length of 19-22 nucleotides. HIV pre-mRNAs are capped quantitatively when elongation complexes are halted at promoter-proximal positions, but capping is much less efficient during unimpeded Pol II elongation. Cotranscriptional capping of HIV mRNA is strongly stimulated by Tat, and this stimulation requires the C-terminal segment of Tat that mediates its direct binding to Mce1. Our findings implicate capping in an elongation checkpoint critical to HIV gene expression.
DOI of Published Version
10.1016/S1097-2765(02)00630-5
Source
Mol Cell. 2002 Sep;10(3):585-97.
Journal/Book/Conference Title
Molecular cell
Related Resources
PubMed ID
12408826
Repository Citation
Chiu Y, Ho CK, Saha N, Schwer B, Shuman S, Rana TM. (2002). Tat stimulates cotranscriptional capping of HIV mRNA. GSBS Student Publications. https://doi.org/10.1016/S1097-2765(02)00630-5. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/212