GSBS Student Publications

Student Author(s)

Kelly N. Hallstrom

GSBS Program

Immunology & Microbiology

UMMS Affiliation

Department of Microbiology and Physiological Systems

Date

6-1-2015

Document Type

Article

Disciplines

Microbiology

Abstract

Salmonella enterica Typhimurium induces intestinal inflammation through the activity of type III secreted effector (T3SE) proteins. Our prior results indicate that the secretion of the T3SE SipA and the ability of SipA to induce epithelial cell responses that lead to induction of polymorphonuclear transepithelial migration are not coupled to its direct delivery into epithelial cells from Salmonella. We therefore tested the hypothesis that SipA interacts with a membrane protein located at the apical surface of intestinal epithelial cells. Employing a split ubiquitin yeast-two-hybrid screen, we identified the tetraspanning membrane protein, p53 effector related to PMP-22 (PERP), as a SipA binding partner. SipA and PERP appear to have intersecting activities as we found PERP to be involved in proinflammatory pathways shown to be regulated by SipA. In sum, our studies reveal a critical role for PERP in the pathogenesis of S. Typhimurium, and for the first time demonstrate that SipA, a T3SE protein, can engage a host protein at the epithelial surface.

Rights and Permissions

© 2014 The Authors.

DOI of Published Version

10.1111/cmi.12406

Source

Cell Microbiol. 2015 Jun;17(6):843-59. doi: 10.1111/cmi.12406. Epub 2015 Jan 24. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

Cellular microbiology

PubMed ID

25486861

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Included in

Microbiology Commons

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