GSBS Student Publications

Title

Subunit connectivity, assembly determinants and architecture of the yeast exocyst complex

Student Author(s)

Caroline M. Duffy; Margaret R. Heider

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Date

1-1-2016

Document Type

Article

Disciplines

Molecular Biology | Structural Biology

Abstract

The exocyst is a hetero-octameric complex that has been proposed to serve as the tethering complex for exocytosis, although it remains poorly understood at the molecular level. Here, we purified endogenous exocyst complexes from Saccharomyces cerevisiae and showed that they are stable and consist of all eight subunits with equal stoichiometry. Using a combination of biochemical and auxin induced-degradation experiments in yeast, we mapped the subunit connectivity, identified two stable four-subunit modules within the octamer and demonstrated that several known exocyst-binding partners are not necessary for exocyst assembly and stability. Furthermore, we visualized the structure of the yeast complex by using negative-stain electron microscopy; our results indicate that the exocyst exists predominantly as a stable, octameric complex with an elongated architecture that suggests that the subunits are contiguous helical bundles packed together into a bundle of long rods.

DOI of Published Version

10.1038/nsmb.3146

Source

Nat Struct Mol Biol. 2016 Jan;23(1):59-66. doi: 10.1038/nsmb.3146. Epub 2015 Dec 14. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

Nature structural and molecular biology

PubMed ID

26656853

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