GSBS Student Publications
GSBS Program
Immunology & Microbiology
Publication Date
2013-09-01
UMMS Affiliation
Department of Medicine
Document Type
Article
Disciplines
Immunology and Infectious Disease | Virology
Abstract
The rabbit is a commonly used animal model in studying antibody responses in HIV/AIDS vaccine development. However, no rabbit monoclonal antibodies (MAbs) have been developed previously to study the epitope-specific antibody responses against HIV-1 envelope (Env) glycoproteins, and little is known about how the rabbit immune system can mimic the human immune system in eliciting such antibodies. Here we present structural analyses of two rabbit MAbs, R56 and R20, against the third variable region (V3) of HIV-1 gp120. R56 recognizes the well-studied immunogenic region in the V3 crown, while R20 targets a less-studied region at the C terminus of V3. By comparison of the Fab/epitope complex structures of these two antibodies raised by immunization with that of the corresponding human antibodies derived from patients chronically infected with HIV-1, we found that rabbit antibodies can recognize immunogenic regions of gp120 and mimic the binding modes of human antibodies. This result can provide new insight into the use of the rabbit as an animal model in AIDS vaccine development.
Rights and Permissions
Publisher PDF posted as allowed by the publisher's author rights policy at http://journals.asm.org/site/misc/ASM_Author_Statement.xhtml.
DOI of Published Version
10.1128/JVI.00843-13
Source
J Virol. 2013 Sep;87(18):10221-31. doi: 10.1128/JVI.00843-13. Epub 2013 Jul 17. Link to article on publisher's site
Journal/Book/Conference Title
Journal of virology
Related Resources
PubMed ID
23864637
Repository Citation
Pan, Ruimin; Sampson, Jared M.; Chen, Yuxin; Vaine, Michael; Wang, Shixia; Lu, Shan; and Kong, Xiang-Peng, "Rabbit anti-HIV-1 monoclonal antibodies raised by immunization can mimic the antigen-binding modes of antibodies derived from HIV-1-infected humans" (2013). GSBS Student Publications. 2015.
https://escholarship.umassmed.edu/gsbs_sp/2015