GSBS Student Publications
GSBS Program
Not applicable
Publication Date
2015-08-27
UMMS Affiliation
Department of Medicine, Division of Cardiovascular Medicine
Document Type
Article
Disciplines
Cellular and Molecular Physiology | Molecular Biology
Abstract
Lipid metabolism in liver is complex. In addition to importing and exporting lipid via lipoproteins, hepatocytes can oxidize lipid via fatty acid oxidation, or alternatively, synthesize new lipid via de novo lipogenesis. The net sum of these pathways is dictated by a number of factors, which in certain disease states leads to fatty liver disease. Excess hepatic lipid accumulation is associated with whole body insulin resistance and coronary heart disease. Tools to study lipid metabolism in hepatocytes are useful to understand the role of hepatic lipid metabolism in certain metabolic disorders. In the liver, hepatocytes regulate the breakdown and synthesis of fatty acids via beta-fatty oxidation and de novo lipogenesis, respectively. Quantifying metabolism in these pathways provides insight into hepatic lipid handling. Unlike in vitro quantification, using primary hepatocytes, making measurements in vivo is technically challenging and resource intensive. Hence, quantifying beta-fatty acid oxidation and de novo lipogenesis in cultured mouse hepatocytes provides a straight forward method to assess hepatocyte lipid handling. Here we describe a method for the isolation of primary mouse hepatocytes, and we demonstrate quantification of beta-fatty acid oxidation and de novo lipogenesis, using radiolabeled substrates.
Keywords
Molecular Biology, Issue 102, Liver, Hepatocyte, Mouse, Fatty Acid, Oxidation, Lipogenesis, Metabolism, Palmitate
Rights and Permissions
Copyright © 2015, Journal of Visualized Experiments. Publisher PDF posted as allowed by the publisher's author rights policy at https://www.jove.com/files/Author_License_Agreement.pdf.
DOI of Published Version
10.3791/52982
Source
J Vis Exp. 2015 Aug 27;(102):e52982. doi: 10.3791/52982. Link to article on publisher's site
Journal/Book/Conference Title
Journal of visualized experiments : JoVE
Related Resources
PubMed ID
26382148
Repository Citation
Akie, Thomas E. and Cooper, Marcus P., "Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes" (2015). GSBS Student Publications. 2004.
https://escholarship.umassmed.edu/gsbs_sp/2004