GSBS Student Publications

Title

Preparation of early Drosophila embryonic fillets (before cuticle deposition)

Student Author(s)

Preethi Ramachandran

GSBS Program

Neuroscience

Publication Date

2010-10-01

UMMS Affiliation

Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Document Type

Article

Disciplines

Cell Biology | Developmental Biology | Laboratory and Basic Science Research | Neuroscience and Neurobiology

Abstract

This article describes the dissection of early Drosophila embryos to generate flat or fillet preparations. For the procedure, a modified chamber or well is designed using glass slides and sealant. Embryos are dissected by removing the chorionic (outer) membrane (performed outside the chamber) and then removing the vitelline (inner) membrane (performed inside the chamber and under saline).

DOI of Published Version

10.1101/pdb.prot5497

Source

Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5497. doi: 10.1101/pdb.prot5497. Link to article on publisher's site.

Journal/Book/Conference Title

Cold Spring Harbor protocols

Related Resources

Link to Article in PubMed

PubMed ID

20889694

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