GSBS Student Publications
Title
The ability of an attaching and effacing pathogen to trigger localized actin assembly contributes to virulence by promoting mucosal attachment
GSBS Program
Immunology & Microbiology
UMMS Affiliation
Department of Microbiology and Physiological Systems; Department of Pathology
Date
9-1-2014
Document Type
Article
Medical Subject Headings
Actins; Animals; Enterohemorrhagic Escherichia coli; Escherichia coli Infections; HeLa Cells; Humans; Intestinal Mucosa; Mice; Shiga Toxin; Virulence
Disciplines
Cell and Developmental Biology | Cell Biology | Microbiology | Pathogenic Microbiology
Abstract
Enterohaemorrhagic Escherichia coli (EHEC) colonizes the intestine and causes bloody diarrhoea and kidney failure by producing Shiga toxin. Upon binding intestinal cells, EHEC triggers a change in host cell shape, generating actin 'pedestals' beneath bound bacteria. To investigate the importance of pedestal formation to disease, we infected genetically engineered mice incapable of supporting pedestal formation by an EHEC-like mouse pathogen, or wild type mice with a mutant of that pathogen incapable of generating pedestals. We found that pedestal formation promotes attachment of bacteria to the intestinal mucosa and vastly increases the severity of Shiga toxin-mediated disease.
Rights and Permissions
Citation: Cell Microbiol. 2014 Sep;16(9):1405-24. doi: 10.1111/cmi.12302. Epub 2014 Jun 2. Link to article on publisher's site
DOI of Published Version
10.1111/cmi.12302
Related Resources
Journal Title
Cellular microbiology
PubMed ID
24780054
Repository Citation
Mallick, Emily M.; Garber, John J.; Vanguri, Vijay K.; Balasubramanian, Sowmya; Blood, Timothy; Clark, Stacie; Vingadassalom, Didier F.; Louissaint, Christopher; McCormick, Beth A.; Snapper, Scott B.; and Leong, John M., "The ability of an attaching and effacing pathogen to trigger localized actin assembly contributes to virulence by promoting mucosal attachment" (2014). GSBS Student Publications. 1943.
https://escholarship.umassmed.edu/gsbs_sp/1943