The ability of an attaching and effacing pathogen to trigger localized actin assembly contributes to virulence by promoting mucosal attachment
Department of Microbiology and Physiological Systems; Department of Pathology
Cell and Developmental Biology | Cell Biology | Microbiology | Pathogenic Microbiology
Enterohaemorrhagic Escherichia coli (EHEC) colonizes the intestine and causes bloody diarrhoea and kidney failure by producing Shiga toxin. Upon binding intestinal cells, EHEC triggers a change in host cell shape, generating actin 'pedestals' beneath bound bacteria. To investigate the importance of pedestal formation to disease, we infected genetically engineered mice incapable of supporting pedestal formation by an EHEC-like mouse pathogen, or wild type mice with a mutant of that pathogen incapable of generating pedestals. We found that pedestal formation promotes attachment of bacteria to the intestinal mucosa and vastly increases the severity of Shiga toxin-mediated disease.
DOI of Published Version
Cell Microbiol. 2014 Sep;16(9):1405-24. doi: 10.1111/cmi.12302. Epub 2014 Jun 2. Link to article on publisher's site
Mallick EM, Garber JJ, Vanguri VK, Balasubramanian S, Blood T, Clark S, Vingadassalom DF, Louissaint C, McCormick BA, Snapper SB, Leong JM. (2014). The ability of an attaching and effacing pathogen to trigger localized actin assembly contributes to virulence by promoting mucosal attachment. Morningside Graduate School of Biomedical Sciences Student Publications. https://doi.org/10.1111/cmi.12302. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/1943